Coding

Part:BBa_K4877017:Design

Designed by: Team iGEM Bielefeld-CeBiTec 2023   Group: iGEM23_Bielefeld-CeBiTec   (2023-10-12)


mNeonGreen flanked by additional SphI and SalI sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 188
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N-terminal SphI and C-terminal SalI sites were added so that the reporter coding sequence can be exchanged when the part is inside an open reading frame. An illegal PstI restriction site was silently mutated.


Source

The sequence was taken from the published pDV06 plasmid by Gayet et al. [1].

[1] Gayet, R.V., Ilia, K., Razavi, S. et al. Autocatalytic base editing for RNA-responsive translational control. Nat Commun 14, 1339 (2023). https://doi.org/10.1038/s41467-023-36851-z

References